Clin Microbiol Rev 1, Hydroxyl radical generation by polymorphonuclear leukocytes measured by electron spin resonance spectroscopy. Lactoferrrin-catalysed hydroxyl radical production. Additional requirement for a chelating agent. Myeloperoxidase as an effective inhibitor of hydroxyl radical production: Implications for the oxidative reactions of neutrophils.
Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus: A spin-trapping study. Pseudomonas and neutrophil products modify transferrin and lactoferrin to create conditions that favor hydroxyl radical formation.
Possible role of bacterial siderophores in inflammation—Iron bound to the pseudomonas siderophore pyochelin can function as a hydroxyl radical catalyst. Bactericidal properties of hydrogen peroxide and copper or iron-containing complex ions in relation to leukocyte function. Spin trapping evidence for myeloperoxidase-dependent hydroxyl radical formation by human neutrophils and monocytes. Free hydroxyl radicals are formed on reaction between the neutrophil-derived species superoxide and hypochlorous acid.
Radiation induced damage in Escherichia coli B: The effects of superoxide radicals and molecular oxygen. Radiation-induced generation of chlorine derivatives in N2O-saturated phosphate buffered saline: Toxic effects on Escherichia coli cells.
Evidence for the generation of an electronic excitation state s in human polymorphonuclear leukocytes and its participation in bactericidal activity. On the question of singlet oxygen production in leucocytes, macrophages and the dismutation of superoxide anion.
Intracellular singlet oxygen generation by phagocytosing neutrophils in response to particles coated with a chemical trap. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. Kinetics of oxidation of tyrosine and dityrosine by myeloperoxidase compounds I and II.
Dityrosine, a specific marker of oxidation, is synthesized by the myeloperoxidase-hydrogen peroxide system of human neutrophils and macrophages. Oxidative inactivation of Escherichia coli by hypochlorous acid. Rates and differentiation of respiratory from other reaction sites. Effects of the putative neutrophil-generated toxin, hypochlorous acid, on membrane permeability and transport systems of Escherichia coli. General mechanism for the bacterial toxicity of hypochlorous acid: Abolition of ATP production.
The inhibition of bacterial growth by hypochlorous acid; possible role in the bacterial activity of phagocytes. Redundant contribution of myeloperoxidase-dependent systems to neutrophil-mediated killing of Escherichia coli. Myeloperoxidase-catalyzed oxidation of chloride and other halides: The role of chloramines.
Chlorination of endogenous amines by isolated neutrophils. Ammonia-dependent bactericidal, cytotoxic and cytolytic activities of the chloramines. Candidacidal activity of the neutrophil myeloperoxidase system can be protected from excess hydrogen peroxide by the presence of ammonium ion.
Formation of reactive nitrogen species during peroxidase-catalyzed oxidation of nitrite: A potential additional mechanism of nitric oxide-dependent toxicity.
Myeloperoxidase-dependent generation of a tyrosine peroxide by neutrophils. Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation. The comparative toxicity of nitric oxide and peroxynitrite to Escherichia coli.
Effect of nitric oxide on staphylococcal killing and interactive effect with superoxide. Formation and release of nitric oxide from human neutrophils and HL cells induced by a chemotactic peptide, platelet activating factor and leukotriene B4.
Kinetics of nitric oxide and hydrogen peroxide production and formation of peroxynitrite during the respiratory burst of human neutrophils. Flow cytometric analysis of nitric oxide production in human neutrophils using dichlorofluorescein diacetate in the presence of calmodulin inhibitor. Rat, mouse and human neutrophils stimulated by a variety of activating agents produce much less nitrite than rodent macrophages.
Human polymorphonuclear leukocytes lack detectable nitric oxide synthase activity. Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria. Reactive nitrogen intermediates and antimicrobial activity: Role of nitrite. Electron microscopic study of phagocytosis of Escherichia coli by human polymorphonuclear leukocytes.
Linkage of azurophil granule secretion in neutrophils to chloride ion transport and endosomal transcytosis. Proton channels, plasma membrane potential, and respiratory burst in human neutrophils. The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH.
Characteristics of the granulocyte chemiluminescence reaction following an interaction between human neutrophils and Salmonella typhimurium bacteria. Phagocytosis following translocation of the the b-cytochrome from the specific granules to the plasma membrane is associated with an increased leakage of reactive oxygen species.
Failure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease. Superoxide production by polymorphonuclear leukocytes A cytochemical approach.
Cytochemical demonstration of hydrogen peroxide in polymorphonuclear phagosomes. H2O2 release from human granulocytes during phagocytosis.
Relationship to superoxide anion formation and cellular catabolism of H2O2: Studies with normal and cytochalasin B-treated cells. Quantitative and temporal characterization of the extracellular hydrogen peroxide pool generated by human neutrophils. The involvement of superoxide and myeloperoxidase in oxygen-dependent bacterial killing. Production of the superoxide adduct of myeloperoxidase compound III by stimulated neutrophils, and its reactivity with H2O2 and chloride.
Superoxide modulates the activity of myeloperoxidase and optimizes the production of hypochlorous acid. Superoxide is an antagonist of anti-inflammatory drugs that inhibit hypochlorous acid production by myeloperoxidase. Iodination by stimulated human neutrophils.
Studies on its stoichiometry, subcellular localization and relevance to microbial killing. Myeloperoxidase-dependent fluorescein chlorination by stimulated neutrophils. Intraphagosomal chlorination dynamics and yields determined using unique fluorescent bacterial mimics. Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils. Role of the phagocyte in host-parasite interactions XII. Hydrogen peroxide-myeloperoxidase bactericidal system in the phagocyte. The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils.
Modification of neutrophil oxidant production with diphenyleneiodonium and its effect on neutrophil function. Leukocyte bactericidal activity in chronic granulomatous disease: Correlation of bacterial hydrogen peroxide production and susceptibility to bacterial killing.
Defective bactericidal activity in myeloperoxidase-deficient human neutrophils. Role of myeloperoxidase mediated antimicrobial systems in intact leukocytes.
Myeloperoxidase: Contribution to the microbicidal activity of intact leukocytes. Role of myeloperoxidase in the killing of Staphylococcus aureus by human neutrophils: Studies with the myeloperoxidase inhibitor salicylhydroxamic acid. The bactericidal effects of the respiratory burst and the myeloperoxidase system isolated in neutrophil cytoplasts. Inhibition of neutrophil killing of Candida albicans pseudohyphae by substances which quench hypochlorous acid and chloramines.
The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. Catalase, superoxide dismutase, and virulence of Staphylococcus aureus. In vitro and in vivo studies with emphasis on staphylococcal-leukocyte interaction. Catalase and superoxide dismutase in Escherichia coli. Roles in resistance to killing by neutrophils.
Role of catalase and superoxide dismutase in the virulence of Listeria monocytogenes. Superoxide dismutase and the resistance of Escherichia coli to phagocytic killing by human neutrophils. Comparison of the sensitivities of Salmonella typhimurium oxyR and katG mutants to killing by human neutrophils.
Superoxide dismutase protects Escherichia coli against killing by human serum. Purification and properties of a unique superoxide dismutase from Nocardia asteroides. Purification of Helicobacter pylori superoxide dismutase and cloning and sequencing of the gene.
Cloning and molecular characterization of Cu,Zn superoxide dismutase from Actinobacillus pleuropneumoniae. Role of superoxide dismutase and catalase as determinants of pathogenicity of Nocardia asteroides: Importance in resistance to microbicidal activities of human polymorphonuclear neutrophils.
Monoclonal antibodies demonstrate that superoxide dismutase contributes to protection of Nocardia asteroides within the intact host.
How important is the myeloperoxidase microbicidal system of phagocytic cells? Chemiluminescence and superoxide production by myeloperoxidase-deficient leukocytes. Myeloperoxidase modulates the phagocytic activity of polymorphonuclear neutrophil leukocytes.
Studies with cells from a myeloperoxidase-deficient patient. Increased degranulation of human myeloperoxidase-deficient polymorphonuclear leucocytes. Hydrogen peroxide utilization in myeloperoxidase-deficient leukocytes: A possible microbicidal control mechanism. Neutrophil activation on biological surfaces: Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. Respiratory burst facilitates the digestion of Escherichia coli killed by polymorphonuclear leukocytes.
Macrophage phagocytosis of aging neutrophils in inflammation: Programmed cell death in the neutrophil leads to its recognition by macrophages. Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production.
Absence of respiratory burst in X-linked chronic granulomatous disease mice leads to abnormalities in both host defense and inflammatory response to aspergillus fumigatus.
Detection of chlorotyrosine in albumin exposed to stimulated human neutrophils. Add comment Close comment form modal. Submit a comment. Comment title. You have entered an invalid code. Although red blood cells RBCs are often described as oxygen carriers, their real function goes far beyond this simplistic image. RBCs also transport numerous macromolecules, the most emblematic of which is ATP, the major source of energy in the organism. Pretty nice read. I wish I could have written something about Pi.
It's my 2nd favorite number value. Also, I don't think Pi is as mysterious as everyone thinks it is. It's just a number with a lot of personalities. And I do believe there is a last digit. While the Perspective is intriguing, the sheer Chutzpah of writing a "manifesto" about a field of modeling with lists of "demands" based on experiments that in many cases are more limited in scope or reality than any physics-based model, is quite astounding.
Neither artificial membrane slabs, nor "live cells" imaged under conditions in which cells have a shabby life that doesn't last long how much of this is due to the mistreatment of the membrane proteins? No experiment, computational or using imaging, is meant to mimic reality, only to probe it based on models and assumptions that can be falsified.
As to the role of the cytoskeleton, what does this tell us about the membrane itself, or the behavior of membrane proteins as individual molecules in their interplay with the membrane? And since this unrelated scaffold differs greatly between cells, what is the "correct matching standard"?
All models are wrong, some computational, or on the stage of a microscope are useful, but in my opinion, self-critical, humble, and rigorous scientific reasoning are preferable to a manifesto in order to foster progress. BPS Blog. Publications BPS Blog.
What drives immune cells to engulf pathogens? Go Back. Tags: BJ cover art immune cells macrophage cells pathogens. Answered by Beth F. Need help with Biology? One to one online tuition can be a great way to brush up on your Biology knowledge. Answered by Joe W. Answered by James K.
0コメント